TCR alpha/beta Antibody (FITC)

SKU: orb154478

Description

Mouse monoclonal antibody conjugated to FITC which recognizes TCR alpha/beta that recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either alpha and beta or gamma. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex.

Images & Validation

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Tested Applications	FC
Reactivity	Primate, Rat
Application Notes	Flow cytometry: Recommended dilution: 1-3 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	R73
Immunogen	Rat T blasts and erythrocytes
Target	TCR alpha/beta
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TCRA/B

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Flow cytometry analysis (surface staining) of rat splenocytes with anti-rat TCR alpha/beta (R73) FITC.

Quick Database Links

Gene Symbol

TCR alpha/beta

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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