Anti-TGFalpha [tAb2]

SKU: orb613874

Description

Rabbit monoclonal antibody to TGFalpha

Research Area

Cancer Biology, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG
Clone No.	tAb2
Immunogen	Biologically active hemocyanin-coupled human TGFalpha.
Target	TGFalpha
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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TGFA, Transforming growth factor A

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Flow-cytometry using the anti-TGF-alpha antibody tAb2 Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (orb613874, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

TGFalpha

UniProt

P01135

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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