TNF alpha Antibody

SKU: orb1293354

Description

The TNF alpha Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against E. coli-expressed, recombinant mouse TNF-α .Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution range	IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Reactivity	Mouse
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Hamster
Clonality	Monoclonal
Clone No.	TN3-19.12
Immunogen	E. coli-expressed, recombinant mouse TNF-α
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Armenian Hamster IgG. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Quality Guarantee

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Quick Database Links

UniProt

P06804

NCBI Gene ID

21926

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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