Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively.
Target	Tumor necrosis factor
Molecular Weight	25 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; Tnf; Tnfa, Tnfsf2

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Anti-TNF alpha Picoband antibody, Western blotting. All lanes: Anti TNF alpha at 0.5 ug/ml. Lane 1: Mouse Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Intestine Tissue Lysate at 50 ug. Predicted bind size: 25 KD. Observed bind size: 25 KD.

Anti-TNF alpha Picoband antibody, Western blotting. All lanes: Anti TNF alpha at 0.5 ug/ml. WB: Recombinant Mouse TNF alpha Protein 0.5 ng. Predicted bind size: 15 KD. Observed bind size: 15 KD.

Quick Database Links

Gene Symbol

Tumor necrosis factor

UniProt

P06804

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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