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APEX1 Antibody
SKU: orb1248020
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | The immunogen for this antibody is: PKRGKKGAVAEDGD-C |
| Target | APEX1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 500 ug/mL |
| Disclaimer | For research use only |
Alternative Names
−APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic), APEX nuclease 1 endonuclease, deoxyribonuclease (apurinic or apyrimidinic), apurinic/apyrimidinic (abasic) endonuclease, redox factor 1
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orb1248020 (4 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
orb1248020 (0.3 ug/ml) staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001632.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
APEX1 Antibody (orb1248020)
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