Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human APEX1 (201-318/318aa)
Target	APEX1
Molecular Weight	36 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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APEX1_HUMAN; DNA-(apurinic or apyrimidinic site) endonuclease; EC:3.1.11.2; APE; APE1; APEX; APX; HAP1; REF1; APEX nuclease(APEN); Apurinic-apyrimidinic endonuclease 1(AP endonuclease 1; APE-1); REF-1; Redox factor-1; DNA-(apurinic or apyrimidinic site) endonuclease, mitochondrial;

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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (APEX1) polyclonal Antibody, Unconjugated (orb10110) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

APEX1

UniProt

P27695

UniProt Details

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No UniProt data available

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Protocol Information

ICC

Immunocytochemistry

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