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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 1
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-100, IF=1:50-200, IP=1:20-50 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 6C19 |
| Immunogen | A synthesized peptide derived from human BIRC3 (400-450/604aa) |
| Target | BIRC3 |
| Molecular Weight | 72 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AIP1; API2; CIAP2; HAIP1; HIAP1; IAP-1; MALT2; MIHC; RNF49; c-IAP2; Api1; HIAP2; IAP1; IAP2; MIAP1; MIAP2; MIHB; cIAP-1; cIAP-2; cIAP1; Birc2; BIRC3_HUMAN; BIRC3; Apoptosis inhibitor 2 (API2); Cellular inhibitor of apoptosis 2 (C-IAP2); IAP homolog C; Inhibitor of apoptosis protein 1 (hIAP-1 | hIAP1); RING finger protein 49; RING-type E3 ubiquitin transferase BIRC3; TNFR2-TRAF-signaling complex protein 1; 2.3.2.27; BIRC3_MOUSE; Inhibitor of apoptosis protein 1 (mIAP1);

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Western blot analysis of Ramos cell lysate. Using API2/BIRC3 (orb2562985) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Quick Database Links
Gene Symbol
BIRC3
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
API2/BIRC3 Recombinant Rabbit Monoclonal Antibody (orb2562985)
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