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ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220)
SKU: orb1939527
Description
Images & Validation
−Item 1 of 3
| Tested Applications | WB |
|---|---|
| Dilution range | WB - 1:1000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 25454 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Quality Guarantee
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All lanes: Anti-Ascl1 (Human C-term) at 1:1000 dilution. Lane 1: human brain lysate. Lane 2: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 25 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of anti-Ascl1 Antibody (C-term D220) in Hela cell line lysates (35 ug/lane). Ascl1 (arrow) was detected using the purified Pab (1:160 dilution).
Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2).
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ASCL1 (Achaete-scute homolog 1) Antibody (C-term D220) (orb1939527)
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