Avian Dopamine (DA) ELISA Kit

SKU: orb782490

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dopamine(DA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dopamine(DA).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian DA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian DA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Aves
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2.5h
Range	15.63-1000 pg/mL
Sensitivity	4.61 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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2-(3,4-Dihydroxyphenyl)ethylamine ELISA Kit, 3,4-Dihydroxyphenethylamine ELISA Kit, 3-Hydroxytyramine ELISA Kit, Intropin ELISA Kit, Revivan ELISA Kit, Oxytyramine ELISA Kit, Dopamine Hydrochloride ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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