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B7-1 Recombinant Protein
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus / Rhesus macaque CTLA-4, His Tag at 2 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque B7-1, Fc Tag with a linear range of 1-8 ng/mL. |
| Target | B7-1 |
| Tag | Fc Tag |
| Molecular Weight | 50.5 kDa |
| Protein Sequence | Val 35 - Asn 242 |
| Purity | >95% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
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ELISA, WB
>95% as determined by SDS-PAGE.
50.9 kDa
HEK293 cells
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
E.coli
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The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide.
Mammalian
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
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Cynomolgus/Rhesus macaque B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
B7-1 Recombinant Protein (orb1227823)
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