SMARCD1 Antibody

SKU: orb1255505

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Description

SMARCD1 Antibody

Research Area

Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	IHC, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of mouse BAF60a (NP_114030.2).
Target	SMARCD1
Molecular Weight	Observed: 58kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, 60 kDa BRG-1/Brm-associated factor subunit A, BRG1-associated factor 60A, BAF60A, Protein D15KZ1, SWI/SNF complex 60 kDa subunit, Smarcd1, Baf60a, D15Kz1

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Western blot analysis of extracts of various cell lines, using Smarcd1 antibody (orb1255505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (orb1255505) (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (orb1255505) (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (orb1255505) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (orb1255505) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

SMARCD1

UniProt

Q96GM5

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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