Bax Antibody (BH3 Domain Specific)

SKU: orb1939544

Description

Research Area

Cancer Biology, Cell Biology, Infectious Disease & Virology, Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 5

Tested Applications	FC, IHC-P, WB
Dilution range	IHC-P - 1:100-500, WB - 1:2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	21184 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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BCL2L4

Quality Guarantee

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All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: THP-1 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: L929 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: Raji whole cell lysate. Lane 2: SH-SH5Y whole cell lysate. Lane 3: HT-1080 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: L929 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: L929 whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Bax Antibody (BH3) at 1:2000 dilution + mouse spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

UniProt

Q07812

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Flow Cytometry

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