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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | ARBC2-1 |
| Immunogen | Recombinant full-length human protein was used as the immunogen for the Bcl2 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC testing of FFPE human melanoma with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min and allow to cool before testing.
IHC testing of FFPE human tonsil with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min and allow to cool before testing.
Western blot testing of human MCF7 cell lysate with Bcl2 antibody (clone ARBC2-1). Routinely observed at 18-22 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Bcl2 Antibody (orb750150)
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