Bcl-x (phospho Thr47) rabbit pAb

SKU: orb769910

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Description

Rabbit polyclonal antibody to Bcl-x (phospho-Thr47)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human BCL-XL around the phosphorylation site of Thr47. AA range:13-62
Molecular Weight	30
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-BCL2L1 antibody, anti-BCL2L antibody, anti-BCLX antibody, anti-Bcl-2-like protein 1 antibody, anti-Bcl2-L-1 antibody, anti-Apoptosis regulator Bcl-X antibody

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Western blot analysis of RAT-MUSLE using Phospho-Bcl-x (T47) antibody. Antibody was diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BCL-XL (Phospho-Thr47) Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BCL-XL (Phospho-Thr47) Antibody. The picture on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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