CD269 Antibody / BCMA / TNFRSF17

SKU: orb1825325

Description

The B cell maturation protein (BCMA) is a type I integral membrane protein that belongs to the tumor necrosis factor receptor (TNF-R) superfamily. It is expressed as a 184 amino acid peptide that is expressed only in mature B-lymphocytes and is located on the cis part of the Golgi apparatus. BCMA shares significant homology with TACI (transmembrane activator) within the cysteine-rich domain. TACI has been shown to bind CAML, which induces activation of NFAT (nuclear factor of activated T cells). Both BCMA and TACI have been shown to bind APRIL and TALL-1, which stimulate B cell proliferation in conjunction with other B-cell activators. When overexpressed, TALL-1 stimulates the development of systemic lupus erythaematosus (SLE).

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the CD269 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2, kappa
Clone No.	BCMA/7254
Immunogen	A recombinant human protein fragment (within amino acids 1-184) was used as the immunogen for the CD269 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human colon carcinoma tissue with CD269 antibody (clone BCMA/7254). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Daudi cell lysate with CD269 antibody (clone BCMA/7254). Expected molecular weight: 20-27 kDa depending on glycosylation level.

Quick Database Links

UniProt

Q02223

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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