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beta Actin antibody
SKU: orb181785
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Description
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−Item 1 of 7
| Tested Applications | ELISA, ICC, IF, IHC-P, WB |
|---|---|
| Dilution range | WB: 1:500, IHC: 1:200, IF/ICC: 1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human beta actin protein (orb84841) was used as immunogen. |
| Target | beta Actin |
| Molecular Weight | 42 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−anti ACTB antibody, anti Actin cytoplasmic 1 antibody, anti Actx antibody, anti b actin antibody, anti b-actin antibody, anti Beta cytoskeletal actin antibody, anti Beta-actin antibody, anti cytoplasmic 1 antibody, anti Melanoma X actin antibody, anti N-terminally processed antibody
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Immunohistochemical staining of paraffin embedded mouse brain tissue using beta actin antibody (primary antibody at 1:200)
IHC-P staining of mouse brain tissue using anti-beta actin (dilution at 1:200)
WB analysis of recombinant human beta actin antibody (dilution of 1:500)
Immunofluorescence analysis of mouse brain tissue using beta actin antibody (dilution of primary antibody - 1:200)
IF analysis of mouse brain tissue using beta actin antibody (dilution of primary antibody at 1:200)
IHC-P image of mouse brain tissue using anti-beta actin (dilution of primary antibody at 1:200)
Immunohistochemical staining of human breast cancer tissue using beta actin antibody (dilution of primary antibody - 1:200)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Li Xu 1, Jie Su 2, Caili Du 3, Hui Liu 4, Wei Lv Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3 Cell Mol Biol (Noisy-le-grand), (2024)
Applications
IF
Reactivity
Rat
Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)
beta Actin antibody (orb181785)
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