Biotinylated Human IL-17RA

SKU: orb2993686

Description

Biotinylated Recombinant Human Interleukin-17 receptor A is produced by our Mammalian expression system and the target gene encoding Leu33-Trp320 is expressed with a 6His, Avi tag at the C-terminus.

Images & Validation

−

Key Properties

−

Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human IL-17F(Cat#orb2995245) at 2μg/ml (100 μl/well)can bind Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686).The ED50 of Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686) is 0.11 ug/ml. Immobilized Anti-Human IL-17RA mAb(Cat#orb2993107) at 2μg/ml (100 μl/well)can bind Human IL-17RA-His-Avi(Cat#orb2993686).The ED50 of Human IL-17RA-His-Avi(Cat#orb2993686) is 0.37 ng/ml.. BLI: Loaded Biotinylated Human IL-17RA-His-Avi(Cat#orb2993686) on HIS1K Biosensor, can bind Biotinylated Human IL-17RA-Fc(Cat#orb2993107) with an affinity constant of 0.18 nM as determined in BLI assay.
Tag	C-6His-Avi
Molecular Weight	Predicted: 36.3 KDa. Observed: 50-80 KDa, reducing conditions
Expression Region	Leu33-Trp320
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

−

Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

−

IL17Rinterleukin-17 receptor A; interleukin 17 receptor A; IL17RA; CD217

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

Q96F46

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols