Biotinylated Human Siglec-10

SKU: orb2993776

Description

Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 10 is produced by our Mammalian expression system and the target gene encoding Met17Thr546 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Anti-Human Siglec10 mAb(Cat#orb2993121) at 1μg/ml (100 μl/well) can bind Biotinylated Human Siglec-10-Fc-Avi(Cat#orb2993776 ) The ED50 of Biotinylated Human Siglec-10-Fc-Avi(Cat#orb2993776 ) is 1.45 ng/ml.. BLI: Loaded Human Siglec-10-Fc-Avi (Cat#orb2993776) on SA Biosensor, can bind Anti-Human Siglec-10 mAb-Fc(Cat#orb2993121) with an affinity constant of -3 nM as determined in BLI assay.
Tag	C-Fc-Avi
Molecular Weight	Predicted: 86.9 KDa. Observed: 100-130 KDa, reducing conditions
Expression Region	Met17Thr546
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SIGLEC10; MGC126774; PRO940; Siglec10; SLG2; sialic acid-binding Ig-like lectin 10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

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UniProt

Q96LC7

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No UniProt data available

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