Biotinylated Human/Cynomolyus CD28

SKU: orb2993831

Description

Biotinylated Recombinant Human T-cell-specific surface glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human/Cynomolyus
Biological Activity	BLI: Loaded Human B7-1-His(Cat#orb2994419) on HIS1K Biosensor, can bind Biotinylated Human/Cynomolgus CD28-Fc-Avi (Cat#orb2993831) with an affinity constant of 9.09 pM as determined in BLI assay. Loaded Biotinylated Human/Cynomolgus CD28-Fc-Avi(Cat#orb2993831) on SA Biosensor, can bind Anti-Human CD28 mAb(Cat#GMP-A063) with an affinity constant of 6.4 pM as determined in BLI assay.
Tag	C-Fc-Avi
Molecular Weight	Predicted: 44.1 KDa. Observed: 60-90 KDa, reducing conditions
Expression Region	Asn19-Pro152
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 20mTris-HCl, 150mM NaCl, pH 8.0.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44

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UniProt

P10747

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