Bovine Collagen Type I protein

SKU: orb750375

Description

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, SDS-PAGE, WB
Application Notes	Bovine Collagen Type I purified protein standard is used as a control for SDS-PAGE, Western Blot, ELISA, immunoprecipitation, and for other immunological assays. Specific conditions should be optimized by user. Collagen type I is recognized by type specific Anti-Collagen antibodies that recognize a native three-dimensional structure.

Key Properties

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Source	Bovine
Biological Origin	Bovine
Target	COL1A1
Purity	Bovine Collagen Type 1 has been prepared from Bovine Placenta and is chromatographically and immunologically pure. Bovine Collagen Type 1 reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. Do not freeze. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.5 M Acetic Acid
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Type I collagen protein, collagen 1 protein, bovine collagen protein, Alpha 1 type I collagen protein, Alpha 2 type I collagen protein, COL1A1 protein, COL1A2 protein, Collagen alpha-1(I) chain protein, Collagen alpha-2 (I) chain protein

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Aggf1 interacts with and modulates SMAD7 activity. (D) Primary HSCs were infected with Ad-V or Ad-AGGF1 and then transfected with either siRNA targeting SMAD7 or scrambled siRNA (SCR). Expression of fibrogenic genes was examined by Western and qPCR (not shown).

Aggf1 suppresses liver fibrosis following injury in mice. (B) C57/BL6 mice were injected via tail vein adenovirus carrying either Aggf1 expression vector (Ad-AGGF1) or an empty vector (Ad-V) followed by CCl4 injection to induce liver injury. Expression of fibrogenic genes was examined by Western and qPCR (not shown).

C57/BL mice were injected peritoneally with chaetocin (25 mg/kg) or DMSO 2 days prior to the LAD procedure. The mice were sacrificed 4 weeks after the surgery. (B) Expression of pro-fibrogenic genes was examined by Western blotting and qPCR (not Shown).

SDS-PAGE Results of Bovine Collagen Type I. Lane 1: Bovine Collagen Type I -Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker Lane 3: Bovine Collagen Type I -Non-Reduced [10 µg]. 4-20% Gel, Coomassie Stained.

Quick Database Links

Gene Symbol

COL1A1

UniProt

P02453

RefSeq (Protein):NP_001029211.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001029211.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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