Brefeldin A

SKU: orb1301107

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Description

Brefeldin A is a macrolide antibiotic and potent ATPase inhibitor that induces tumor cell differentiation and apoptosis while also inhibiting autophagy. It is widely used in cancer research to study intracellular transport mechanisms in both cellular and animal models.

Research Area

Cell Biology

Images & Validation

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Key Properties

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CAS Number	20350-15-6
MW	280.4
Purity	>98%
Formula	C₁₆H₂₄O₄
SMILES	[H][C@@]12C[C@H](O)C[C@@]1([H])[C@H](O)\C=C\C(=O)O[C@@H](C)CCC\C=C\2
Target	ER/Golgi
Solubility	Soluble in DMSO (up to 100 mg/ml) or in Ethanol (up to 25 mg/ml).

Bioactivity

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Target IC50	ATPase (HCT 116 cells):0.2 μM\|HepG2 cells proliferation:239.1 nM\|A549 cells proliferation:101.2 nM\|HeLa cells proliferation:171.9 nM
In Vivo	In HF4.9 and HF28RA cells, Brefeldin A (25 ng/mL) completely inhibits cell growth. Similarly, in HF1A3 cells, Brefeldin A (75 ng/mL) fully inhibits cell growth. Read more...
In Vitro	METHODS: Tumor cells HL60, K562 and HT-29 were treated with Brefeldin A (2 μM) for 72 h. DNA fragments were detected by DNA filter elution assay. RESULTS: Brefeldin A induced DNA fragmentation with different kinetics. intact DNA fragments were observed in HL60 cells within 15 h, whereas 48-72 h was required for K562 and HT-29 cells. METHODS: Human breast cancer cells MDA-MB-231 were treated with Brefeldin A (0.05-1 μg/mL) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: PARP cleavage, a hallmark event of cell death, could be detected in Brefeldin A-treated suspension MDA-MB-231 cells. Read more...
Cell Research	HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference) Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cas9, Brefeldin A, Cyanein, CRISPR/Cas9, CRISPR, Decumbin, BFA, Ascotoxin, ATPase (HCT 116), Autophagy, Antibiotic, inhibit, Mitochondrial Autophagy, Mitophagy, Inhibitor, Herpes simplex virus, HSV

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Compound Identifiers

PubChem CID

133640201 ↗

Key Properties

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No computed properties available.

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Brefeldin A (orb1301107)

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Applications:	ELISA, IF, IHC, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, ICC, IF, IHC, IHC-P, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	IHC, IP, WB
Predicted Reactivity:	Bovine, Rat
Reactivity:	Human, Mouse
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IHC, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Brefeldin A

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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Brefeldin A (orb1301107)

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