Calnexin Antibody

SKU: orb385943

Description

It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Images & Validation

−

Item 1 of 7

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the Desmin antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	CANX/1541
Immunogen	A partial human recombinant protein was used as the immunogen for the Calnexin antibody.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.

IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0 for 10-20 min.

Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.

Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.

Immunofluorescent staining of human HeLa cells with Calnexin antibody (green) (clone CANX/1541). RedDot used as nuclear counterstain (red).

SDS-PAGE analysis of purified, BSA-free Calnexin antibody (clone CANX/1541) as confirmation of integrity and purity.

Quick Database Links

UniProt

P27824

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols