Calnexin Antibody

SKU: orb20292

Description

Goat polyclonal antibody to Calnexin

Images & Validation

−

Item 1 of 10

Tested Applications	ELISA, IF, IHC, WB
Dilution range	ELISA: 1:128000, WB: 0.1-1μg/ml, IHC-P: 5μg/ml, IF/ICC: 10μg/ml
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Mouse, Porcine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/ml.

Key Properties

−

Host	Goat
Clonality	Polyclonal
Target	Calnexin
Molecular Weight	67.6
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

−

anti Calnexin antibody, anti CANX antibody, anti CNX antibody, anti IP90 antibody, anti IP 90 antibody, anti IP-90 antibody, anti Calnexin-CT antibody, anti Calnexin-NT antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

orb20292 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

orb20292 (6µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20292 Immunofluorescence analysis of paraformaldehyde fixed LNCaP cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and membrane staining.

orb20292 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining.

orb20292 (0.5µg/ml) staining of LNCaP (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb20292 (0.3µg/ml) staining of Human Cerebellum (A) and (0.5ug/ml) Rat Brain (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of Human Cerebellum (A) and Colorectal cancer (B) lysate using Calnexin antibody

Western blot analysis of CaCo-2 (A) and NIH3T3 (B) cell lysate using Calnexin antibody

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells using Calnexin antibody.

Immunohistochemical staining of Human Testis using Calnexin antibody

Quick Database Links

Gene Symbol

Calnexin

NCBI Gene ID

821

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols