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Description
Research Area
Molecular Biology
Images & Validation
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Item 1 of 2
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1µg/mL |
| Reactivity | Bacteria |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein. |
| Target | cas9 |
| Purity | Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-Cas9 antibody biotin conjugation, biotin conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_269215.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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