Cas 9 Antibody Biotin Conjugated

SKU: orb420487

Description

Cas 9 antibody (Biotin)

Images & Validation

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Tested Applications	ELISA, IF, WB
Dilution Range	ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1µg/mL
Reactivity	Bacteria
Application Notes	Cas9 Biotin Conjugated Antibody has been tested for use in ELISA and suitable for Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
Purity	Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1 mg/mL
Expiration Date	12 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Cas9 antibody biotin conjugation, biotin conjugated rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

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Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1 µg/ml. Secondary: Donkey anti-Rabbit at 1 µg/ml, staining seen in red. Bottom images show GFP stain overlay.

Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15 µg per lane. Primary Antibody cas9 used at 1 µg/ml using orb348637 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40000 for 30 min at room temp. Expect: 158kDa.

Quick Database Links

UniProt

Q99ZW2

RefSeq (Protein):NP_269215.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_269215.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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