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Catenin-α E/N rabbit pAb
SKU: orb764736
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Catenin-alpha1. AA range:857-906 |
| Molecular Weight | 100 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-CTNNA1 antibody, anti-Catenin alpha-1 antibody, anti-Alpha E-catenin antibody, anti-Cadherin-associated protein antibody, anti-Renal carcinoma antigen NY-REN-13 antibody, anti-CTNNA2 antibody, anti-CAPR antibody, anti-Catenin alpha-2 antibody, anti-Alpha N-catenin antibody, anti-Alpha-catenin-related protein antibody
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Immunofluorescence analysis of Hela cell. 1, Catenin-α E/N Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of various cells using Catenin-α E/N Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Catenin-alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using Catenin-alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.
Quick Database Links
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Catenin-α E/N rabbit pAb (orb764736)
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