Cattle Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

SKU: orb780935

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1A(BMPR1A).

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle BMPR1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle BMPR1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle BMPR1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle BMPR1A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	Tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.52 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD292 ELISA Kit, ACVRLK3 ELISA Kit, ALK3 ELISA Kit, SKR5 ELISA Kit, BMPR1-A ELISA Kit, Activin A Receptor, Type Ii-Like Kinase 3 ELISA Kit, Activin Receptor-Like Kinase 3 ELISA Kit, Serine/Threonine-Protein Kinase Receptor R5 ELISA Kit

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Cattle Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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