Cattle Ceruloplasmin (CP) ELISA Kit

SKU: orb866944

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Description

Sandwich Animal CP ELISA Kit

Research Area

Infectious Disease & Virology, Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle CP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Animal
Sample Types	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	15.6-1000 ng/mL
Sensitivity	5.58 ng/mL
Concentration	1000 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CP2 ELISA Kit, CER ELISA Kit, Caeruloplasmin ELISA Kit, Ferroxidase ELISA Kit, Oxygen Oxidoreductase ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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