CAV1/Caveolin 1 Antibody

SKU: orb1537707

This product can be custom made

Description

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	IF (10 µg/ml), IHC-P (2.5 µg/ml), Peptide-ELISA (1:8000), WB (0 - 0.01 µg/ml)
Reactivity	Human, Monkey
Application Notes	Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Read more...

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-DELSEKQVYDAH, from the internal region of the protein sequence according to NP_001744.2NP_001166366.1.
Target	CAV1 / Caveolin 1
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CAV1, Caveolin-1, CGL3, CAV, MSTP085, VIP21, BSCL3

CAV1 Antibody [orb11600]

Applications:	IF, IHC-Fr, IHC-P, WB
Reactivity:	Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
Species/Host:	Goat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 100 μg

Item 1 of 1
CAV1/Caveolin 1 Antibody [orb1533864]
Applications:
IHC-P
Reactivity:
Bovine, Canine, Equine, Guinea pig, Human, Mammal, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 50 μg

Quality Guarantee

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Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

CAV1 / Caveolin 1 antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

CAV1 / Caveolin 1 antibody (0.003 µg/ml) staining of A431 (A) and (0.001 ug/ml) A549 cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

CAV1 / Caveolin 1 antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

CAV1 / Caveolin 1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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