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CAV1 Antibody
SKU: orb1248004
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Canine, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | The immunogen for this antibody is: C-DELSEKQVYDAH |
| Target | CAV1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Concentration | 500 ug/mL |
| Disclaimer | For research use only |
Alternative Names
−CAV1, caveolin 1, caveolae protein, 22kDa , HGNC:1527, CAV, MSTP085, VIP21, caveolae protein, 22-kD, caveolin 1, caveolin 1 caveolae protein, 22kD, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32, BSCL3, CGL3, MSTP085
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orb1248004 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1248004 (0.003 ug/ml) staining of A431 (A) and (0.001 ug/ml) A549 cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248004 (0.003 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1248004 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and membrane staining.
orb1248004 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi and cytoplasmic staining.
orb1248004 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001744.2, NP_001166366.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CAV1 Antibody (orb1248004)
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