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CCAR1 Antibody
SKU: orb1260339
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2). |
| Target | CCAR1 |
| Molecular Weight | Observed: 155kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−CCAR1, cell-cycle and apoptosis regulatory protein 1, OTTHUMP00000019709, RP11-437A18.1, MGC44628, CARP1, CARP-1, cell division cycle and apoptosis regulator 1, CCAR1, DIS
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Western blot analysis of extracts of various cell lines, using CCAR1 antibody (orb1260339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using CCAR1 antibody (orb1260339). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug CCAR1 antibody (orb1260339). Western blot was performed from the immunoprecipitate using CCAR1 antibody (orb1260339) at a dilution of 1:500.
Quick Database Links
Gene Symbol
CCAR1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
CCAR1 Antibody (orb1260339)
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