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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | CD28 |
| Tag | His Tag |
| MW | 16.9 kDa |
| Purity | >90% as determined by SDS-PAGE. |
| Protein Sequence | Asn 20 - Lys 149 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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ELISA, FACS, IF, IHC-P, WB
Human
Rabbit
Recombinant
Unconjugated
100 μgRecombinant Human /Cynomolgus CD28 Protein [orb2995095]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.7 KDa. Observed: 30-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human/Cynomolgus CD28 Protein (Biotin) [orb2993831]
Biotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 44.1 KDa. Observed: 60-90 KDa, reducing conditions
20 μg, 100 μgRecombinant Human /Cynomolgus CD28 Protein [orb2993961]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 42.3 KDa. Observed: 40-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human /Cynomolgus CD28 Protein [orb2994044]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 41.7 KDa. Observed: 40-75 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μg

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Mouse CD28, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_031668.3 |
|---|
Documents Download
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Protocol Information
CD28 Recombinant Protein (orb1227698)
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