CD3 Antibody (clone CD3-12)

SKU: orb1735043

Description

Images & Validation

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Item 1 of 1

Tested Applications	FC, IHC, IHC-Fr, IHC-P, WB
Dilution Range	Flo (1:50 - 1:100), IHC, IHC-Fr (1:1000), IHC-P (10 µg/ml), WB (1:50 - 1:100)
Reactivity	Human
Application Notes	Further information: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Flow Cytometry: 1:50-1:100; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:1000.

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG1
Clone No.	CD3-12
Immunogen	Synthetic peptide sequence derived from cytoplasmic epitope of CD3.
Purification	Protein G purified from tissue culture supernatant
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 0.09% Sodium Azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Flow Cytometry

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