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CD3 antibody

SKU: orb2276524

Description

CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

Tested ApplicationsIHC
Dilution Range1:100-1:200
ReactivityHuman
Application Notes
Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells). Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining. Cellular Localization: Cell surface. Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

HostRabbit
ClonalityMonoclonal
IsotypeIgG
Clone No.MSVA-003R
ImmunogenA synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex

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CD3 antibody

Appendix mucosa In the appendix numerous CD3 positive T lymphocytes occur in the epithelium the lamina propria and in germinal centres.

CD3 antibody

Diffuse large B cell lymphoma infiltrated by numerous CD3 positive non neoplastic lymphocytes.

CD3 antibody

Squamous cell carcinoma containing numerous CD3 positive T lymphocytes.

UniProt Details

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Protocol Information

IHC
Immunohistochemistry
View Protocol

CD3 antibody (orb2276524)

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0.1 ml
¥ 6,630.00
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