CD3-ZETA/CD247 Antibody

SKU: orb1248109

Description

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Reactivity	Human
Application Notes	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16-17kDa band observed in lysates of cell lines Jurkat and MOLT4 (calculated MW of 18.6kDa according to NP_000725.1). Recommended concentration: 0.1-1ug/ml.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: C-KNPQEGLYNELQKD
Target	CD3-ZETA / CD247
Molecular Weight	Approx 16 + 17kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Disclaimer	For research use only

Alternative Names

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CD247, CD247 molecule, CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ, CD247 antigen, zeta subunit, CD3Z antigen, zeta polypeptide (TiT3 complex), CD3zeta chain, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, TCR zeta chain

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orb1248109 (0.1 ug/ml) staining of Jurkat (A) and (0.3 ug/ml) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

CD3-ZETA / CD247

UniProt

P20963

RefSeq (Protein):NP_932170.1, NP_000725.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_932170.1, NP_000725.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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