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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized HIV GP120 (HIV), His Tag at 10 ug/mL (100 uL/well) can bind Rhesus macaque CD4, Fc Tag with a linear range of 0.04-0.31 ug/mL. |
| Target | CD4 |
| Tag | Fc Tag |
| MW | 67 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Lys 26 - Trp 390 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD4,CD4mut,LEU3
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Rhesus macaque CD4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD4 Recombinant Protein (orb1227632)
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