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CD5 Antibody
SKU: orb1151521
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | rC5/6429 |
| Immunogen | Recombinant full-length protein corresponding to human CD5 was used as the immunogen for the recombinant CD5 antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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SDS-PAGE analysis of purified, BSA-free recombinant CD5 antibody (clone rC5/6429) as confirmation of integrity and purity.
Western blot testing of human Jurkat cell lysate with recombinant CD5 antibody (clone rC5/6429). Observed molecular weight: 55~67 kDa depending on glycosylation level.
IHC staining of FFPE human tonsil tissue with recombinant CD5 antibody (clone rC5/6429). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue using recombinant CD5 antibody (clone rC5/6429) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CD5 Antibody (orb1151521)
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