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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0. 04 - 0. 2 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized rhB7-2 / CD86 Fc Chimera at 2μg/mL (100μL/well) can bind Biotinylated rh CTLA4, His. Avi Tag with a linear range of 0. 1 - 1μg/mL. |
| Target | CD86 |
| Tag | C-Fc Tag |
| MW | 51.5 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Leu 26 - Pro 247 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD86, B7-2, B70, CD28LG2, LAB72, MGC34413
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The purity of rh CD86 Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
CD86 Recombinant Protein (orb1228136)
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