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CD8A Antibody
SKU: orb1151692
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P |
|---|---|
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rCD8/6590 |
| Immunogen | A portion of amino acids 135-235 of CD8 alpha protein was used as the immunogen for the recombinant CD8A antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human lymph node with recombinant CD8A antibody (clone rCD8/6590). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD8A antibody (clone rCD8/6590). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD8A antibody (clone rCD8/6590) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD8A antibody (rCD8/6590) as confirmation of integrity and purity.
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CD8A Antibody (orb1151692)
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