CDC37 Antibody (HRP)

SKU: orb151600

Description

Rabbit polyclonal to CDC37 (HRP). Hsp90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies.

Images & Validation

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Tested Applications	ICC, IF, IHC
Dilution range	WB (1:2000), ICC/IF (1:200)
Reactivity	Human
Application Notes	A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Native human Cdc37, full length
Target	CDC37
Molecular Weight	44.5kDa
Purification	Protein A Purified
Conjugation	HRP

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
Concentration	1.68 mg/ml
Disclaimer	For research use only

Alternative Names

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CDC37, Hsp90 co-chaperone Cdc37, Cell division control protein 37, Hsp90 chaperone protein kinase-targeting subunit, CDC37_HUMAN, CDC37A, p50, p50Cdc37, S. cerevisiae hypothetical protein CDC37

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Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of CDC37 protein using Rabbit Anti-CDC37 Polyclonal Antibody. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:2000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-CDC37 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-CDC37 Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-CDC37 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

Quick Database Links

Gene Symbol

CDC37

UniProt

Q16543

NCBI Gene ID

11140

RefSeq (Protein):NP_008996.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_008996.1

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Protocol Information

IHC

Immunohistochemistry

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Immunofluorescence

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ICC

Immunocytochemistry

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