CFSE

SKU: orb1300451

Description

CFSE

Images & Validation

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Key Properties

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CAS Number	150347-59-4
MW	557.46
Purity	98.98%
Formula	C₂₉H₁₉NO₁₁
SMILES	CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C22OC(=O)c3cc(ccc23)C(=O)ON2C(=O)CCC2=O)c1.CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C22OC(=O)c3ccc(cc23)C(=O)ON2C(=O)CCC2=O)c1
Target	Others
Solubility	DMSO:93 mg/mL (166.83 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (5.92 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

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In Vivo	METHODS: CFSE-labeled CD8+OT-I T cells were injected intravenously int the tail of C56BL/6J mice, followed by intravenous injection of OVA (20 μg), and CFSE profiles were measured three days later. Results: Most o the cells fell within 7 CFSE peaks, indicating tha the cells had undergone up to 6 divisions. METHODS: To label thymocytes in vivo CFSE (10 μM) was injected int the thymic lobes of anesthetized C56B-/- mice. Results: CFSE labeled a representative sample of All thymocyte subpopulations and these cells migrated to peripheral lymphoid organs at a rate of approximately 2-3 x 10^6 cells/day. They ente the lymph nodes on day 1 post injection and remain there for at least 21 days, while turnover is faster in the spleen. Read more...
In Vitro	METHODS: 1 mL of cells and CFSE (5 μM in 110 μL PBS) were flipped and mixed to labe the cells. CFSE-labeled CD8+OT-I T cells were cultured with dendritic cells pulsed with varying amounts of OVA for 3 days, and CFSE profiles were examined using Flow Cytometry. Results: CD8+ T cells divided 1-3 times according to the CFSE dilution peak, and more T cells divided at higher antigen concentration . METHODS: Human erythroleukemia cell line K562, mouse lymphoma cell line YAC-1, human breast cancer cell line MCF-7, and human melanoma cell line A375 were treated with CFSE (1-10 μM) for 1-6 h. Cell death was detected by Flow Cytometry. Results: CFSE was non-toxic to the cells, a the cell death rate due to CFSE labeling was less than 5%. Read more...
Cell Research	Instructions: I. Solution preparation 1. Preparation of Other solution: Take 1 mg CFDA-SE and dissolve it in 0.1794 mL DMSO to obtain 10 mM CFDA-SE Other solution. Note the Other solution is recommended to be stored at -20℃ or -80℃ away from light to avoid repeated freezing and thawing. 2. Preparation of working solution: Use pure DMEM to dilut the Other solution, usually with a concentration of 1-1 μM. Note: Please adjus the concentration of CFDA-SE working solution according to actual conditions. II. Cell staining 1. Cell type: 1) Suspended cells: Centrifuge at 4℃, 1000 g for 3-5 minutes, discar the supernatant. Wash with PBS twice, 5 minutes Each time. 2) Adherent cells: Discar the cell culture medium, add trypsin to dissociat the cells, and make a Single cell suspension. Centrifuge at 4℃, 1000 g for 3-5 minutes, discar the supernatant. Wash with PBS twice, 5 minutes Each time. 3. Add 1 mL CFDA-SE working solution and incubate at room temperature for 30 minutes. 4. Centrifuge at 400 g for 3-4 minutes at 4°C 5. Wash twice with PBS, 5 minutes Each time. 6. Resuspend cells in serum-free cell culture medium or PBS and detect by fluorescence microscopy or flow cytometry. Read more...

Storage & Handling

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Storage	keep away from direct sunlight,store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Disclaimer	For research use only

Alternative Names

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inhibit, Inhibitor, 5(6)-CFDA N-succinmidyl ester, 5(6)-Carboxyfluorescein diacetate succinimidyl ester, Carboxyfluorescein succinimidyl ester, CFDA-SE, CFSE

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Compound Identifiers

PubChem CID

16211581 ↗

Key Properties

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No computed properties available.

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CFSE

Description

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Key Properties

Bioactivity

Storage & Handling

Alternative Names

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