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Cleaved-Caspase-1 p20 (D210) rabbit pAb
SKU: orb770550
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution range | WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase-1. AA range:161-210 |
| Molecular Weight | 25 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-CASP1 antibody, anti-IL1BC antibody, anti-IL1BCE antibody, anti-Caspase-1 antibody, anti-Caspase 1 antibody, anti-CASP-1 antibody, anti-Interleukin-1 beta convertase antibody, anti-IL-1BC antibody, anti-Interleukin-1 beta-converting enzyme antibody, anti-ICE antibody, anti-IL-1 beta-converting enzyme antibody, anti-p45 antibody
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Cleaved-Caspase-1 (D210) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Cleaved-Caspase-1 p20 (D210) rabbit pAb (orb770550)
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