COL1A1 Rabbit Polyclonal Antibody

SKU: orb1294293

Description

Rabbit polyclonal antibody to COL1A1

Images & Validation

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Item 1 of 8

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:1000-1:1500, Immunofluorescence 1:300–1:500, Immunohistochemistry (Paraffin) 1:100–1:300
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / encompassing a sequence within the C-terminus region.
Target	COL1A1 / Collagen I
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Anti-COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min. Cells were stained with orb1294293 anti-COL1A1 / Collagen I antibody (red) at 1:400 and 4°C.

COL1A1 / Collagen I antibody - at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

COL1A1 / Collagen I antibody - at 1/250 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1294293 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling COL1A1 / Collagen I with orb1294293 at 1/150.

Immunohistochemical analysis of paraffin embedded Mouse tissue labeling COL1A1 / Collagen I with orb1294293 at 1/100.

Quick Database Links

Gene Symbol

COL1A1 / Collagen I

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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