Collagen I Mouse Monoclonal Antibody

SKU: orb2563523

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Description

Research Area

Cell Biology, Collagen, Osteogenesis

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-Fr, IHC-P
Dilution range	IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	3C2
Immunogen	KLH conjugated synthetic peptide derived from human Collagen I
Target	COL1A1
Molecular Weight	139 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Collagen type I; alpha 1 type I collagen; alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein.

Quality Guarantee

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Paraformaldehyde-fixed, paraffin embedded Human Uterus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Prostate, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Collagen I Monoclonal Antibody, Unconjugated (orb2563523) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Quick Database Links

Gene Symbol

COL1A1

UniProt

P02452

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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