Collagen III Mouse Monoclonal Antibody

SKU: orb500868

Description

Research Area

Cell Biology, Chondrogenesis, Collagen, Osteogenesis

Images & Validation

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Item 1 of 1

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:500-1000, IHC-F=1:500-1000, ICC/IF=1:100-200, IF=1:500-1000
Reactivity	Human
Predicted Reactivity	Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	7B6
Immunogen	Recombinant human Collagen III protein
Target	COL3A1
Molecular Weight	117 kDa
Purification	Affinity purified by Protein G
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1(Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen; CO3A1_HUMAN; Collagen alpha-1(III) chain; Type III collagen; type III preprocollagen alpha 1 chain.

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Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-Collagen III (orb500868) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 117 kD.

Quick Database Links

Gene Symbol

COL3A1

UniProt

P02461

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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