Collagen III Rabbit Polyclonal Antibody

SKU: orb10437

This product can be custom made

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Description

Collagen III Rabbit Polyclonal Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 3

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Gallus, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (1301-1400/1466aa)
Target	COL3A1
Molecular Weight	150-200 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EDS4A; EDSVASC; PMGEDSV; Col3a-1; Tsk-2; Tsk2; CO3A1_BOVIN; COL3A1; CO3A1_HUMAN; CO3A1_MOUSE; CO3A1_RAT; collagen type III alpha 1 chain; Ehlers-Danlos syndrome type IV, autosomal dominant; collagen, type III, alpha 1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-Collagen III (orb10437) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kDa, Observed band size: 130 kDa.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen III (orb10437) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120/170 kD, Observed band size: 170 kD.

Sample: Raji (human) cell Lysate at 40 ug, Primary: Anti-Collagen III (orb10437) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 140 kD.