Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	9E4
Immunogen	KLH conjugated synthetic peptide derived from human Collagen IV
Target	COL4A1
Molecular Weight	161 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS (pH7.2) with 40% Glycerol, 0.05% rAlbumin and 0.02% Proclin300.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Arresten; Canstatin; COL4A1; HANAC; ICH; POREN1; Collagen alpha 1(IV) Chain; Collagen IV alpha 1 Polypeptide; Collagen Of Basement Membrane alpha 1 Chain; Collagen Of Basement Membrane alpha 2 Chain; Collagen Type IV alpha 1; DKFZp686I14213; FLJ22259; collagen alpha-1(IV) chain preproprotein; collagen alpha-1(IV) chain preproprotein; Col4a1 protein; collagen of basement membrane, alpha-1 chain; collagen IV, alpha-1 polypeptide; collagen alpha-1(IV) chain; COL4A1 NC1 domain; Collagen Ⅳ; Collagen Type Ⅳ.

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Collagen IV Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Collagen IV) Monoclonal Antibody, Unconjugated (orb1974642) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Collagen IV) Monoclonal Antibody, Unconjugated (orb1974642) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

COL4A1

UniProt

P02462

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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