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Collagen Type I, human Antibody

SKU: orb867293

Description

Rabbit polyclonal antibody to Collagen Type I

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

Tested ApplicationsELISA, IF, IHC-P, RIA, WB
Dilution Range(purified, lyophilized) IFA 1:80, IHC(P) 1:200 - 1:600, ELISA >1:200 (OD>500)
Application Notes
Positive Control: Human skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ImmunogenPurified collagen type I from human skin
Purificationaffinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentrationapp. 1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
RIA
Radioimmunoassay
View Protocol

Collagen Type I, human Antibody (orb867293)

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0.1 ml
¥ 10,790.00
DispatchUsually dispatched within 5-10 working days
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