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CREB-1 Rabbit Polyclonal Antibody
SKU: orb10458
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Canine, Gallus, Porcine, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CREB-1 (101-150/341aa) |
| Target | CREB1 |
| Molecular Weight | 37 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Active transcription factor CREB; cAMP response element binding protein; cAMP responsive element binding protein 1; CREB1; CREB 1; CREB1; MGC9284; Transactivator protein; CREB; CREB1_HUMAN.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CREB-1) Polyclonal Antibody, Unconjugated (orb10458) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-CREB-1 (orb10458) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.
Sample: Hela Cell Lysate at 40 ug, Primary:Anti-CREB-1 (orb10458) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.
Tissue/Cell: human cervical carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CREB-1 Polyclonal Antibody, Unconjugated (orb10458) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
CREB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
CREB-1 Rabbit Polyclonal Antibody (orb10458)
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