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CSF2 Antibody
SKU: orb1270661
Featured
Description
Research Area
Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This CSF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-85 amino acids from the Central region of human CSF2. |
| Target | CSF2 |
| Molecular Weight | 16 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF
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Western blot analysis in HL60 cell line lysates (35 ug/lane).
CSF2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CSF2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
CSF2 Antibody (orb1270661)
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